Application

Phospholipid assay kit has been used in biochemical analysis and phospholipid quantitation.

Features and Benefits

Compatible with high-throughput handling systems.

General description

Phospholipids are a class of lipids which constitute a major component of cell membranes and play important roles in signal transduction. Most phospholipids contain one diglyceride, a phosphate group, and one choline. In this assay, phospholipids (such as lecithin, lysolecithin and sphingomyelin) are enzymatically hydrolyzed to choline which is determined using choline oxidase and a H₂O₂-specific dye. This results in a colorimetric (570 nm)/ fluorometric (λ_ex = 530/λ_em = 585 nm) product directly proportional to the phospholipid concentration in the sample. The range of linear detection is 3 - 200 µM for colorimetric assays and 0.6 - 20 µM for fluorometric assays.

Principle

In this assay, phospholipids (such as lecithin, lysolecithin and sphingomyelin) are enzymatically hydrolyzed to choline which is determined using choline oxidase and a H₂O₂-specific dye. This results in a colorimetric (570 nm)/ fluorometric (λ_ex = 530/λ_em = 585 nm) product directly proportional to the phospholipid concentration in the sample. The range of linear detection is 3 - 200 µM for colorimetric assays and 0.6 - 20 µM for fluorometric assays.

Suitability

Suitable for the detection of phospholipids in biological samples